OPTIMIZATION OF ENZYMATIC HYDROLYSIS CONDITIONS FOR ANTIBACTERIAL PEPTIDES PRODUCTION AGAINST PANTOEA SPP. CAUSING RICE LEAF BLIGHT

Main Article Content

Siti Norazura Jamal
https://orcid.org/0000-0001-8717-5433
Dr. Dhilia Udie Lamasudin
https://orcid.org/0000-0002-2568-0909
Dr. Belal J. Muhialdin
Assoc. Prof. Dr. Noor Baity Saidi
https://orcid.org/0000-0002-0715-2133
Dr. Lai Kok Song
https://orcid.org/0000-0002-1887-2232
Assoc. Prof. Dr. Mohd Termizi Yusof
https://orcid.org/0000-0002-5241-3443

Abstract

The Central Composite Design (CCD) within the Response Surface Methodology (RSM) was applied to optimize the enzymatic hydrolysis process. This process used Alcalase® to hydrolyze Bactronophorus thoracites protein with the goal of maximizing its antimicrobial effects. Four distinct parameters were identified as independent variables: pH (A: 8.5–10.5), temperature (B: 45–65 °C), hydrolysis time (C: 120–360 min), and enzyme-to-substrate ratio (D: 1.45%–2.65% w/v). Meanwhile, the antimicrobial activity was chosen as the response variable, specifically against Pantoea ananatis (Y1) and Pantoea stewartii (Y2). According to the findings, the constructed quadratic polynomial model showed a significant correlation with the experimental data, as evidenced by the coefficient of determination (R2) values for antimicrobial activity: Y1 being 0.9893 (p < 0.0001) and Y2 at 0.9848 (p < 0.0001). Optimal antimicrobial activity for Bactronophorus thoracites protein hydrolysates (BTPH) was recorded at 46.748% against P. ananatis and 40.768% against P. stewartii. This result was observed under the optimal conditions of pH 9.5, temperature 55ºC, hydrolysis duration of 240 minutes, and 2.05% w/v enzyme-to-substrate ratio. There was a notable alignment between the actual and predicted values from our models, with the Residual Standard Error (RSE) values falling under 5%. Furthermore, the established Minimum Inhibitory Concentration (MIC) was 250µg/mL, and the Minimum Bactericidal Concentration (MBC) was 500µg/mL for both P. ananatis and P. stewartii. In conclusion, the findings suggest that the refined BTPH has great promise as an effective bioactive component for agricultural use.

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How to Cite
Jamal, S. N., Udie Lamasudin, D., Muhialdin, B. J. ., Saidi, N. B. ., Kok Song , L. ., & Yusof, M. T. . (2024). OPTIMIZATION OF ENZYMATIC HYDROLYSIS CONDITIONS FOR ANTIBACTERIAL PEPTIDES PRODUCTION AGAINST PANTOEA SPP. CAUSING RICE LEAF BLIGHT . Malaysian Journal of Science, 43(3), 1–12. https://doi.org/10.22452/mjs.vol43no3.1
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Original Articles

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